Thiogalactoside transacetylase of the lactose operon as an enzyme for detoxification.
نویسندگان
چکیده
Thigalactoside transacetylase, the lacA gene product, confers selective advantage to cells of Escherichia coli K-12 growing on beta-galactosides in the presence of non-metabolizable analogues.
منابع مشابه
Sequential transcription of the genes of the lactose operon and its regulation by protein synthesis.
The initial kinetics of induction and the steady state rates of synthesis of ,&galactosidase and thiogalactoside transacetylase in Escherichia coli were examined under a variety of conditions. Kinetic experiments with 5-fluorouracil and carbon starvation suggest that the z gene is transcribed into messenger ribonucleic acid (mRNA) before the a gene. In general, when protein synthesis was impair...
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Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight extent, and does not affect the differential rate of synthesis of beta-galactosid...
متن کاملCatabolite repression of the lac operon. Separt epressionof two enzymes.
1. Catabolite repression of beta-galactosidase and of thiogalactoside transacetylase was studied in several strains of Escherichia coli K 12, in an attempt to show whether a single site within the structural genes of the lac operon co-ordinately controls translational repression for the two enzymes. In all experiments the rate of synthesis of the enzymes was compared in glycerol-minimal medium ...
متن کاملThiogalactoside transacetylase. Physical and chemical studies of subunit structure.
The subunit size of thiogalactoside transacetylase from Escherichia coli ML 308 in 6 M guanidinium chloride measured by sedimentation equilibrium in the ultracentrifuge was found to be 29,700 =t 2,000, about half the molecular weight of the native protein. The two chains were identical by estimation of the number of peptides obtained after treatment with trypsin, BrCN, and with both reagents in...
متن کاملDNA sequence of the lactose operon: the lacA gene and the transcriptional termination region.
The lac operon of Escherichia coli spans approximately 5300 base pairs and includes the lacZ, lacY, and lacA genes in addition to the operator, promoter, and transcription termination regions. We report here the sequence of the lacA gene and the region distal to it, confirming the sequence of thiogalactoside transacetylase and completing the sequence of the lac operon. The lacA gene is characte...
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عنوان ژورنال:
- Journal of bacteriology
دوره 128 1 شماره
صفحات -
تاریخ انتشار 1976